Öz
Amaç
İnsan lökosit antijeni (HLA) molekülleri yabancı antijenleri immün hücrelere sundukları ve immün yanıtı başlatmaları açısından önemli moleküllerdir. Bu sebeple, hastalıklarla ilişkili oldukları gibi solid organ ve hematopoetik hücre nakillerinde kritik rol oynarlar. Bu çalışmada, Türk popülasyonundaki HLA allel ve 3 lokus haplotip frekanslarının belirlenmesi amaçlanmıştır. Bu çalışma Türkiye’de yapılmış en büyük popülasyonu içermektedir.
Yöntem
Bu çalışmaya 6.039 kemik iliği vericisi dahil edilmiştir. HLA tiplemesi sekans spesifik oligonükleotit prob ve sekans spesifik primer yöntemleriyle gerçekleştirilmiştir. Akraba kişiler hariç tutulduktan sonra toplamda 1222 A, 1229 B, 930 DRB1, 102 C, 227 DQB1 ve 163 DQA1 allel çiftiyle birlikte 336 ABDR haplotip çifti Arlequin v3.5 Programı ve R paket program ile analiz edilmiştir. Sonuçlar komşu ülkelerdeki frekanslarla karşılaştırılmıştır.
Bulgular
Popülasyonumuzda en sık saptanan HLA alleleri A*02, B*35, C*07, DRB1*11, DQA1*01 ve DQB1*03 olarak bulundu. En sık prevalansa sahip ABDRB1 haplotipi ise A*24 B*35 DRB1*11 olarak belirlendi. Bütün lokuslar için popülasyonumuz Hardy-Weinberg eşitliğine uymaktaydı. Bağlantı eşitsizliği analizine göre A-B, A-DRB1 ve B-DRB1 lokusları arasında güçlü bir bağlantı olduğunu gösterdi. Her iki istatistiksel analiz sonucu da aynı çıktı.
Sonuç
Bulgular komşu ülkelerle benzer oranda çıkmıştır. Bu bulgular ilaç keşfi, hastalık tedavisi ve organ nakli gibi birçok terapötik uygulamayı destekleyici niteliktedir. Bu istatistiksel analizler için Arlequin ve R programlarının uygun olduğu görülmüştür.
Anahtar Kelimeler: HLA, allel frekansı, haplotip frekansı
Kaynakça
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